課程資訊
課程名稱
系統代謝學
Systems Analysis on Metabolomics 
開課學期
104-2 
授課對象
電機資訊學院  生醫電子與資訊學研究所  
授課教師
曾宇鳳 
課號
CSIE5153 
課程識別碼
922EU3820 
班次
 
學分
全/半年
半年 
必/選修
選修 
上課時間
星期四6,7,8(13:20~16:20) 
上課地點
 
備註
本課程以英語授課。教室:基因體中心5樓討論室。與郭錦樺合開
總人數上限:15人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1042CSIE5153_SYSMETA 
課程簡介影片
 
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課程概述

Metabolomics is the "systematic study of the unique chemical fingerprints that specific cellular processes leave behind" - specifically, the study of their small-molecule metabolite profiles. The metabolome represents the collection of all metabolites in a biological organism, which are the end products of its gene expression. Thus, while mRNA gene expression data and proteomic analyses do not tell the whole story of what might be happening in a cell/organ/organism, metabolic profiling can give an instantaneous snapshot of the physiology of that cell/organ/organism. 

課程目標
Metabolomics is the "systematic study of the unique chemical fingerprints that specific cellular processes leave behind" - specifically, the study of their small-molecule metabolite profiles. The metabolome represents the collection of all metabolites in a biological organism, which are the end products of its gene expression. Thus, while mRNA gene expression data and proteomic analyses do not tell the whole story of what might be happening in a cell/organ/organism, metabolic profiling can give an instantaneous snapshot of the physiology of that cell/organ/organism. 
課程要求
 
預期每週課後學習時數
 
Office Hours
另約時間 
指定閱讀
 
參考書目
1. Silas G. Villas-Boas, Jens Nielsen, Jorn Smedsgaard, Michael A. E. Hansen,
Ute Roessner-Tunali, Metabolome Analysis: An Introduction, Wiley, 2007
2. Seetharaman Vaidyanathan and George G. Harrigan, Metabolome analyses:
Strategies for system biology, Springer Science, 2005
3. Howard Mark and Jerry Workman, Jr., 2007, Chemometrics in Spectroscopy,
Academic Press
4. M. Tomita and T. Nishioka, Metabolomics: The Frontier of Systems Biology,
Springer Tokyo, 2005
5. William J. Griffiths, Metabolomics, Metabonomics and Metabolite Profiling,
Baker & Taylor, 2008
 
評量方式
(僅供參考)
 
No.
項目
百分比
說明
1. 
Midterm 
50% 
 
2. 
Final 
50% 
 
 
課程進度
週次
日期
單元主題
第1週
2/25  Introduction to metabolomics 
第2週
3/3  Sampling and Sample Preparation 
第3週
3/10  Metabolome analysis
Principles of chromatography
 
第4週
3/17  Mass spectrometry 
第5週
3/24  LC-MS  
第6週
3/31  GC-MS 
第7週
4/7  Capillary electrophoresis 
第8週
4/14  Nuclear magnetic resonance spectrometry 
第9週
4/21  Midterm exam 
第10週
4/28  Basic Chemometrics 
第11週
5/5  Mass Spectrum analysis 
第12週
5/12  Nuclear magnetic resonance spectrum 
第13週
5/19  1D and 2D NMR Spectroscopy: From Metabolic Fingerprinting to Profiling 
第14週
5/26  New Developments in Multi-dimensional Mass Spectrometry Based Shotgun Lipidomics 
第15週
6/2  Neutral Lipidomics and Mass Spectrometry 
第16週
6/9  Dragon Boat Festival(No Class) 
第17週
6/16  Metabonomics and Global Systems Biology 
第18週
6/23  Final